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 Name:
Dr. P. K. Chakrabarty,
Principal Scientist (BioTechnology) and Head, Crop Improvement
 eMail:
 Qualification
 Ph.D.- Plant Pathology
 Years of experience

 23 Years
Contact Address
Central Institute for Cotton Research,
Post Bag No. 2, Shankar Nagar PO , Nagpur-440010
Phone: 07103-275549 ext. 318 (office), 09850329275 (Cell)


Academic qualification

Institution/Place

Degree awarded

Year

Percent Marks/ G.P.A / Division

Division

Award/ Prize/ Distinction

Central School , CBSE, New Delhi

Higher Secondary

1976

67.33 %

First

Distinction in Chemistry

Panjab University Chandigarh

B.Sc. (Medical)

1979

67.16 %

First

Distinction in Zoology

HPKVV, Palampur

M.Sc. Agril. (Mycology & Pl. Pathology)

1982

3.82/4.00 (76.00 %)

First

Awarded Univ. Merit Fellowship  

University of Horticulture and Forestry, Solan.

Ph.D. (Plant Pathology)

1987

3.91/4.00 (78.20 %)

First

Awarded UNDP Senior Research Fellowship.

Study Overseas

Place

Period

Research work done

Assignment

University of Florida , Gainesville, FL-32611, USA

2001-2003

Functional genomics of bacterial plant pathogens, Signal transduction pathways for disease resistance, cloning Disease resistance genes and development of transgenic plants.

Post doctoral Fellow

University of Missouri , Columbia MO - 65211, USA

2002

Cloning and characterization of a global regulatory gene from Pseudomonas syringae pv. tomato and functional genomics

Post doctoral Fellow

University of Florida , Gainesville, FL-32611, USA

1994 –1996

 

Molecular basis of pathogenicity and host-specificity of Xanthomonas malvacearum.

DBT Overseas Associate (Long-term)

Key Research Achievements

  • Developed Xanthomonas malvacearum detection kit, applied for Patent.
  • Developed PCR detection kit for detection of cotton leaf curl virus infection.
  • Developed PCR diagnostic kits for rapid detection of 5 major fungal pathogens of cotton.
  • Identified CAPS Marker to differentiate Ramularia areola strains infecting diploid cotton from tetraploid cotton.
  • Developed first race 18 specific RFLP marker in Xanthomonas malvacearum .
  • First report of plasmid borne nature of pathogenicity and EPS genes in Xanthomonas malvacearum.
  • Cloned and characterized a pathogenicity gene, pthN (AF016221), from Xanthomonas malvacearum after A. tumefaciens.
  • Associated with Development of Bt-Transgenic diploid ( G. arboreum, RG8) and tetraploid (G. hirsutum, BN-Bt) cotton.
  • Developed two plasmid vectors to create double stranded RNA interference constructs for use in functional genomics and disease resistant plants.
  • Cloned transgenic cotton with enhanced expression of an unique class I chitinase gene from cotton.

Awards/ honour

  • Fellow of the Maharashtra Academy of Sciences elected in Oct 2007
  • KPV Menon Gold Medal for best research paper for 2003-04, Indian Phytopathological Society, New Delhi , Feb 21, 2004.
  • Post doctoral Fellow, University of Florida , Gainesville , FL- 32611 , USA , March 2001-March 2002 and July 2002-Feb 2003.
  • Post doctoral Fellow, University of Missouri , Columbia , MO - 65211, USA , April 2002- June 2002.
  • DBT Overseas Fellowship, Dept. of Biotechnology, Govt. of India , for Post-doctoral Research at the University of Florida , Gainesville, FL-36211, USA , October 1994-June 1996.
  • MJ Narasimhan Award, Indian Phytopathological Society, New Delhi, 1997-1998.
  • INSA Visiting Scientist Fellowship
  • UNDP Fellowship for Doctoral Research, University of Horticulture and Forestry, Solan, H.P., India , 1983-1986.
  • University Merit Fellowship, for M.Sc (Agriculture), HPKVV, Palampur, H.P., India , 1980-1982.

Research Experience in various institutions

S.No

Position

Institution

Duration

1

Head, Division of Crop Improvement

Central Institute for Cotton Research, Nagpur

Aug 21, 2009- continuing

2

Principal Scientist (Biotechnology)

Central Institute for Cotton Research, Nagpur

2005- Aug 20, 2009

3

Senior Scientist
(Plant Pathology)

Central Institute for Cotton Research, Nagpur

1998-2005

4

Scientist Sr. Scale (Plant Pathology)

Central Institute for Cotton Research, Nagpur

1991- 1998

5

Scientist S-1
(Plant Pathology)

Central Institute for Cotton Research, Nagpur

1986- 1991

Research Experience

  • Principal Investigator, Identification of Molecular markers and tagging genes for Bacterial blight resistance, 2008- continued
  • Principal Investigator, Engineering virus resistant cotton through dsRNAi-mediated targeting of cotton leaf curl virus, 2007 –continued.
  • Principal Investigator, DBT sponsored project on DNA based diagnostic methods to identify biotype diversity in race 18 of Xanthomonas axonopodis pv. malvacearum and commercialization of a PCR detection kit from 2004- 2007.
  • Principal Investigator of Project on Development of molecular diagnostic tools for detection and differentiation of races and biotypes of pathogens and pests of cotton, under Technology Mission on Cotton (ICAR), March 2003-2007.
  • Functional genomics of bacterial plant pathogens and Cloning disease resistance gene locus from G. hirsutum, University of Florida, Gainesville, USA, July 2002- March 2003.
  • Signal transduction Pathways-mediated by the rice receptor-like kinase XA21”, University of Florida , Gainesville , USA , March 2001 – March 2002.
  • Cloning and characterization of a global regulatory gene from Pseudomonas syringae pv. tomato , University of Missouri , Columbia , MO - 65211, USA , April 2002- June 2002.
  • Principal Investigator, DBT sponsored project on “Development of molecular diagnostic tools for detection and differentiation of races of Xam ”, 1999– 2002.
  • Co-PI, NATP project on Management of cotton leaf curl virus disease and mass multiplication of predators, parasites and insect pathogens, CICR, Nagpur 1999-2003.
  • Project Leader “Molecular basis of pathogenicity and race-specificity in Xam and characterization of its antagonists”, CICR, Nagpur , 1994 – continued.
  • Molecular basis of pathogenicity and host-specificity in Xam ”, University of Florida , Gainesville , USA , October 1994 – June 1996.
  • Worked in the World Bank sponsored project on “Development of cotton resistant to biotic stresses through genetic engineering”, CICR, Nagpur , 1992 – 1994.
  • Plasmid-borne phenotypes in Xam strains, March 30 – June 29, 1992, University of Madras , Madras , India , as Visiting Scientist under SERC scheme of DST.
  • Project leader on studies on “Biochemical basis of resistance in cotton against foliar diseases”, CICR, Nagpur , 1988 – 1994.
  • Co-Principal Investigator, New wilt of cotton, CICR, Nagpur , 1986-1991.

Other professional recognitions

  • Member, Editorial board of Cotton Research Davelopment Association, Hisar 2007-2010.
  • DBT nominee in Biosafety committee of Institutes involved transgenic Research.
  • President, Indian Phytopathological Society (West Zone) from 2005-06
  • Editor (Bacteriology Section), Indian Phytopathological Society, 2001-2003

Publications

Research Papers

Books Chapter

General Articles

Research Reports

Conference/ Symposia

Gene sequences  

Patents

37

17

5

7

48

39

1 (applied)

Membership of Professional Societies

  • Life member of three prestigious Scientific Societies viz.
  • Indian Phytopathological Society, New Delhi
  • Indian Society of Cotton Improvement, Mumbai
  • Cotton Research and Development Association, Hisar.

Title of project

Funding agency

Duration

 

Position

Cost of project
(in Rupees)

Development, validation, utilization and/ or commercialization of bio-pesticides and bio-inoculants

TMC

2006-2012

PI

 

Identification of Molecular markers and tagging genes for Bacterial blight resistance

DBT

2008-2011

PI

35.00 lakh

Engineering virus resistant cotton through dsRNAi-mediated targeting of cotton leaf curl virus

DBT

2007-2010

PI

40.00 lakhs

DNA based diagnostic methods to identify biotype diversity in race 18 of Xanthomonas axonopodis pv. malvacearum and commercialization of a PCR detection kit

DBT

2004-2007

PI

20.00 lakhs

Development of diagnostic tools for differentiation of biotypes/ races of pests and pathogens of cotton

ICAR/ TMC- Mini Mission I

2003-2007

PI

65.00 lakhs

Signal transduction pathway of disease resistance, functional genomics & Cloning of bacterial blight resistance genes form cotton

NSF/UF
(In Plant Path. Dept. UF ,USA )

2001-2004

RA

Part of NSF grant of USA

Development of sensitive molecular diagnostic tools for rapid detection and differentiation of races of Xanthomonas malvacearum .

DBT

1999 –2002

PI

36 lakh

Management of cotton leaf curl virus disease and mass multiplication of predators, parasites and

NATP

1999-2002

Co-PI

80 lakh
(Multi-centre)

Molecular basis of pathogenicity and host-specificity of X. malvacearum

DBT/ UF

1994-1996

PI/RA

Overseas associateship

Molecular basis of pathogenicity and race- specificity of Xanthomonas malvacearum and characterization of its antagonists.

ICAR

1994-continuing

PI

Instt. Budget

Plasmid-borne phenotypes in strains of Xanthomonas malvacearum strains

DST

March 30 – June 29, 1992

PI

DST/SERC Associateship

Development of cotton resistant to biotic stresses through genetic engineering

World Bank

1992–1994

Co-PI

40 lakh

Biochemical basis of disease resistance

ICAR

1988-1994

PI

Instt. budget

New wilt disease of cotton

ICAR

1986-1991

Co-PI

Instt. Budget

Genes cloned, sequenced and deposited in GenBank at NCBI (www.ncbi. nlm.nih.gov)

SNo. Description of genes

GenBank Accession

  • Cotton leaf curl virus isolate CLCuV-S coat protein (AV1) gene
  • Xanthomonas campestris PthN ( pthN ) gene
  • G.hirsutum Ghi-RGA-A9 NBS resistance protein-like protein gene
  • G.hirsutum Ghi_RGA-B2 NBS resistance protein-like protein gene
  • G.hirsutum Ghi_RGA-C1 NBS resistance protein-like protein gene
  • G.hirsutum Ghi_RGA-D1 NBS resistance protein-like protein gene
  • Alternaria species Back to A. dianthicola ribosomal RNA gene
  • Drechslera australiensis ribosomal RNA gene, partial sequence
  • Drechslera australiensis ribosomal RNA gene, partial sequence
  • Drechslera australiensis ribosomal RNA gene, partial sequence
  • Alternaria strain PBN4 ribosomal RNA gene, partial sequence
  • Alternaria aff. longipes ribosomal RNA gene, partial sequence
  • Alternaria longipes ribosomal RNA gene, partial sequence
  • Phoma helianthi ribosomal RNA gene, partial sequence
  • Alternaria alternata ribosomal RNA gene, partial sequence
  • Alternaria alternata ribosomal RNA gene, partial sequence
  • Alternaria macrospora ribosomal RNA gene, partial sequence
  • Alternaria helianthi ribosomal RNA gene, partial sequence
  • Alternaria brassicae ribosomal RNA gene, partial sequence
  • Alternaria porri ribosomal RNA gene, partial sequence
  • Rhizoctonia bataticola ribosomal RNA gene, partial sequence
  • Rhizoctonia solani ribosomal RNA gene, partial sequence
  • Alternaria citri ribosomal RNA gene, partial sequence
  • Alternaria solani ribosomal RNA gene sequence, partial sequence
  • Xanthomonas. axonopodis pv. malvacearum 16S RNA gene, sequence
  • Mycosphaerella areola ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Mycosphaerella areola, ribosomal RNA gene, partial sequence
  • Myrothecium roridum , ribosomal RNA gene, partial sequence

(AY577460).
(AF016221).
(AY705376).
(AY705377).(AY705378).(AY705379).(AY923859).(AY923860).(AY923861).(AY923862).(AY923863).(DQ156337).(DQ156338).(DQ156339).(DQ156340).(DQ156341).(DQ156342).(DQ156343).(DQ156344).(DQ156345).(DQ339102).(DQ339103).(DQ339104).(EU315064).(DQ414745).(DQ459073).(DQ459074).(DQ459075).(DQ459076).(DQ459077).(DQ459078).(DQ459079).(DQ459080).(DQ459081).(DQ459082).(DQ459083).(DQ459084).(DQ631897).(EU927366).

Salient Research Achievements

1. General Plant Pathology

Extensive investigations on New wilt disease of cotton showed that the malady is a physiological disorder that usually manifests when there is sudden downpour following a dry spell. Documentation of symptoms, factors affecting onset of the malady, ways of ameliorating the disorder was reported ( Cot. et Fib. Trop. 46:207-215 ) . Late season Phoma blight a new disease of cotton was reported for the first time. Detailed symptoms of the disease with various characteristics features were published ( Indian J. Agric. Sci. 61: 223-224 ). The outbreak of a serious disease on silk cotton was responsible for destruction of large number of trees that were planted throughout the farm of the Institute. The disease is characterized by drying of the smaller branches from tip downwards and development of canker on the main trunks. Infection spreads throughout the trees and caused extensive necrosis, drying and death of the fully-grown trees within a short period. The disease is caused by Colletotrichum gloeosporioides. The characteristic symptoms of the disease along with remedial measures are reported (Indian J. Agric. Sci. 63: 599- 601 ) . Some important Biochemical markers for grey mildew resistance in diploid cotton were identified. The resistant cotton were characterized not only by their higher constitutive levels of PAL and the phenolics catalyzed by the enzyme, but also their inherent rapid inducing ability when invaded by the pathogen
( Indian Phytopath. 55: 69-75 ).

2. Molecular basis of pathogenicity of Xanthomonas malvacearum

Extensive studies on molecular basis of pathogenicity and host specificity of Xanthomonas axonopodis pv. malvacearum (Xam), the foliar blight pathogen of cotton is done. Genes governing pigmentation, exopolysaccharide production and pathogenicity in Xam strain are plasmid borne ( Phytopathology 85: 1156; Can. J. Microbiol. 41: 740-745 ). We were successful in cloning and characterizing two pathogenicity genes pthN and pthN2 ( Phytopathology 87: 1160-1167 ). 3.6 kb pthN is completely sequences and the sequence is available in GenBank ( Acc.# AF016221 ). Detailed information generated out of my work on cloning, characterization and sequencing of these pathogenicity genes from a strain of Xam and delineation of its function lead to understanding of some vital aspects of cotton-Xam interactions. These genes belonged to the family of Xanthomonas avr/pth genes, to which several avr genes from wide array of Xanthomonads belongs. The genes cloned by us from Xam strain are perhaps the first genes cloned as pathogenicity genes unlike all other members, which were cloned as avr genes. We have done detailed analysis of these genes from wide range of xanthomonads all of which share several common features (LRR repeat motifs, Leucine zipper, NLSs) like the plant R genes. We have also established through generation of chimeric constructs (by swapping the repeat motifs of the pathogenicity gene pthN , with those from the corresponding repeat regions of the avirulence gene (avrb6) that the central 102-bp repeat region of these avr/pth members are critical for governing host-specific phenotypes, for e.g., HR or water-soaking symptoms. The molecular work on various aspects of Xanthomonas malvacearum are published and presented in various international and National journals and presented in several International symposia.

3. Development of Diagnostic tools for pathogen detection

a. Detection of Xanthomonas malvacearum

The sequence of pthN has been extensively exploited for development of diagnostic tool for detection and differentiation of its strains.

PCR detection and Southern hybridization :

Highly sensitive PCR detection protocol is developed for detection of all strains of Xanthomonas malvacearum that infects cotton. The primers delineate a 0.4 kb DNA fragment from all strains of this pathogen irrespective, of their geographic area of origin or race affiliation. The PCR protocol is rapid and throughput and can detect pathogen in the infected tissue within 1.30 h ( Indian J. Agric. Sci. 75: 524-27 ). A ready to use PCR kit is being developed for commercialization. A Southern hybidization protocol is also developed that detects only the Xanthomonas malvacearum strains of cotton but not other xanthomonads.

Race specific RFLP markers :

Conventional method of identification of races of Xanthomonas malvacearum is laborious and time consuming. We have developed RFLP marker, based upon the number of avr/ pth gene family members, to identify race 18, the most virulent race of this pathogen.

Detection of biotypes within race 18 strains :

Pathological and molecular analysis of Xanthomonas malvacearum strains by Rep-PCR DNA finger printing RFLP, RAPD and plasmid profiles confirmed for the first time existence of at least eight biotypes within race 18 ( Indian Phytopath. 57: 335, KPV Menon Gold medal for Best Paper).

b. Detection of CLCuV

PCR detection :

A pair of primer is designed to detect CLCuV in infected cotton plants as well as symptomless host by amplification of a 0.7 kb coat protein (CP) gene of the virus. The PCR protocol is simple and can be accomplished within 1.44 h (Indian J. Agric. Sci. 75: 524-27 ) .

Immunodiagnostic kit :

The CP gene is amplified from infected plants, cloned and sequenced ( AY577460 ). The sequence analysis of CLCuV-S strain revealed variability in CP gene of other Indian strains of CLCuV. The gene has been cloned and expressed in E. coli . Expressed of fusion protein is ascertained by Western blotting. Research is aimed at developing rapid immunodiagnostic kit for detection of CLCuV in infected cotton as well as symptomless hosts (Indian Phytopath. 58: 2005 )

Twig Imprint blot :

A new and simple tissue imprint blotting protocol for detection of CLCuV infection is developed. The twig imprints of the infected and healthy plants were obtained on the NC membrane. Virus infected plants can be observed by chromogenic detection using anti-CaLCuV (Cabbage leaf curl virus) as primary antibody and goat antirabbit-AP conjugate as secondary antibody (Indian Phytopath. 58: 2005 ).

c. Detection of fungal pathogens

Ribosomal genes and the spacers between them provide attractive targets for molecular detection and phylogenetic studies because they occur in high copy numbers, possess conserved as well as variable sequences and can be amplified and sequenced with universal primers based on their conserved sequences. Considerably greater sequence variation is found in internal transcribed spacer region between the rRNA genes within a rRNA repeat unit. With the objective to develop PCR based diagnostic methods that could enable differential but specific amplification of some economically important fungal pathogens including species of Alternaria and some pathogens of cotton, rDNA sequences were cloned using primers (ITS 1 & 4 and ITS 4 & 5) based on conserved regions of the ribosomal RNA genes. Using the primers rDNA regions of 500 to 650 bp comprising of rDNA genes and internal transcribed spacer regions of thirty two strains of plant pathogenic fungal species were amplified (Pl see genes cloned). Unique stretches of variable rDNA sequences were used for designing primers that can specifically detect 5 major pathogens of cotton including R. bataticola, R. solani, Ramularia areola, Myrothecium roridum and Alternaria macrospora ( World Cotton Research Conference-4, 2007, Phytopathology, communicated) and two pathogens A. helianthi and Drechslera australiensis of sunflower (Euro J. Plant Pathol, communicated, 2009). Besides, CAPS marker for differentiaton of strains of R areola infecting diploid and tetraploid cotton were identified ( Best Paper, International Conference on Toxic Exposure related Biomarker: Genomes & Health Effects, National Environmental Engineering Research Institute, Nagpur , Jan 10-11, 2008 ).

4. Functional genomics of disease resistance genes

Extensive studies to understand the mechanism of signal transduction of Xa21-mediated diseases resistance in rice against Xanthomonas oryzae is done. Blight resistance gene Xa21 encodes a serine-threonine protein kinase that causes autophosphorylation leading to binding of several proteins and triggering downward signaling events in the process of development of HR. At least 7 downstream proteins bind with Xa21; two of them are XB3 and XB2. The molecular mechanism of XB3-Xa21 interaction was demonstrated using protein-protein interaction by yeast dihybrid system. Co-immuno-precipitation of XB3 with Xa21 was demonstrated by inmmuno-capturing followed by western blotting using antibody raised against XB3. Analysis of transgenic rice co-transformed with Xa-21 and a double stranded RNA interference construct of XB3 (dsRNAi-XB3) was done using a series of experiments using Western blotting, Northern analysis, quantitatative PCR, PCR and growth curve analysis of the pathogen. Through dsRNAi-XB3 mediated silencing of XB3 gene in Xa21 transgenic rice, it was established that XB3, governed the abundance of Xa21 protein and hence the resistance against bacterial blight. Interference of XB3 in rice results in the reduction of XA21 protein and a compromised resistance against X. oryzae ( Plant Cell 18: 3635-46). While studying the general molecular mechanisms of disease resistance, a family of genes encoding, ankyrin-ring protein was cloned from Arabidopsis thaliana ( AAR genes) c-DNA library. Two such genes named AAR5 and AAR6 orthologous to rice XB3 were cloned. dsRNAi interference constructs directed towards these two genes were also made to affect gene silencing and delineation of phenotypes encoded by these genes. The functional genomics revealed that the AAR5 (named Xbat-32 in the publication) regulates development of lateral roots in Arabidopsis ( Plant Journal 40: 960-1006 ).

5. Biotechnological approaches for disease resistance

Two bacterial blight immune cotton genotypes (IM216 and S295) possessing multiracial resistance against all existing races of X. malvacearum, in India were identified , maintained and are being used to develop molecular markers for tagging bacterial blight resistance (BBR) genes . Six isogenic lines that differ with respect to single R genes, viz., B2 , B3, B4, B6, B7 and BIn3 effective against specific strains of X. malvacearum in Acala44 (susceptible) background, were developed and maintained. Resistance genes analogues (RGAs) are amplified and cloned from Gossypium hirsutum IM216 (source of all BBR genes) using four sets of primers designed on the basis of conserved sequence of Plant disease resistance (R) genes (Pl. see genes cloned AY-705376-79). RGAs were used as molecular marker (DNA probe) for surveying polymorphism among six BBR genes. A polymorphic RFLP marker for Bin3 is identified ( ICGI, 2004:52 ). Pyramiding of several R genes of variable specificities may provide broad spectrum and sustainable resistance against various diseases.

6. Molecular markers for BBR genes

Besides using RGAs, we initiated work on development of molecular marker for tagging genes for bacterial blight resistance from two immune sources G. hirsutum cotton, IM216 (from USA) and S295 (from Australia). We have initiated the survey of polymorphism between parents with contrasting phenotypes using microsatellite markers. F2 mapping population of a cross between Acala44 (Susceptible) and IM216 (Immune) has been raised and used for development and validation of markers. The markers would be useful in screening for blight resistance, map-based cloning and gene-pyramiding (DBT Project grant/ 2008-2011/BT/PR/9625/AGR/02/467) .

7. Pest resistant transgenic cotton

Bt transgenic G. hirsutum and G. arboreum cotton :

Associated with development of Bt- transgenic Bikaneri Narma ( G. hirsutum ), the first Bt-variety of cotton released for commercial cultivation. I characterized the transgenic plants for gene integration and performed biosafety analysis of Bt-cotton rhizosphere microflora. A robust protocol for transformation and tissue culture regeneration of diploid cotton was developed. This efficient method was used for development of first ever Bt-tansgenic diploid cotton ( G arboreum ) cv RG8. The cry1AC gene has been successfully transformed and characterized. Copy number of the gene has also been ascertained (Plant Mol Biol Reptr, 27:2009).

8. Novel strategies to engineer disease resistance

Novel antimicrobial genes viz., viral sequences (in the form of hairpin RNA), R gene ( Xa21 ), PR protein (chitinase), RGAs, etc. have been cloned for deployment for disease resistance by genetic engineering. These novel strategies together with conventional breeding would lead to robust and sustainable disease and pest management strategies.

Resistance against Bacterial resistance :

Xa-21, a broad spectrum bacterial blight resistance R gene from rice is cloned in binary vector, transformed in A. tumefaciens EHA 101 and is being transformed in cotton. The transgenic cotton is expected to be resistant against X. malvacearum , since the pathogen shares the same avr/pth gene family members as of the rice bacterial pathogen.

Resistance against Fungal resistance :

A unique 1.3 kb class I chitinase gene is cloned from Gossypium hirsutum. The gene unique to G. hirsutum cotton is introduced into G. arboreum cotton (RG-8) by Agrobacterium-mediated transformation. The transgenic plants are being characterized for enhanced expression and resistance against fungal pathogens ( PhD thesis guided ).

Resistance against virus disease (CLCuV) :

>Two double-stranded RNA generating plasmids pKSB-Gus (3.8 kb) and pKSB-Int (3.1 kb) developed for use in RNA interference and development of RNAi-mediated CLCuV resistant transgenic cotton. Using these plasmids Five sets of dsRNAi construct to target 3 regions of DNA A (CP gene, Movement protein) and 2 regions of ?-DNA (BC1 and BV4) components of CLCuV generated. These constructs are being used for transformation in cotton to develop RNAi mediated CLCuV resistant transgenic cotton (DBT Project grant/2007-2010/ BT/PR5259/AGR/02/273).

List of Publications

A. Research Papers

  • Chakrabarty, P.K. 1990. Disease syndrome of Curd Rot of Cauliflower (Brassica oleracea convar botrytis var. botrytis). Indian J. Agric. Sci. 60: 779-80.
  • Chakrabarty, P.K. 1993. Chemical management of Curd rot complex of Cauliflower (Brassica oleracea convar botrytis var. botrytis). Indian J. Agric. Sci. 63: 50-55.
  • Chakrabarty, P.K. and Bambawale, O.M. 1991. Detached hypocotyl method- an efficient technique for screening beans for anthracnose. Indian Phytopath.44: 76-77.
  • Chakrabarty, P.K. and Mayee, C.D. 2004. Recent Approaches to Manage Diseases of Cotton. J. Indian Soc. Cotton Improv. 29:65-79.
  • Chakrabarty, P.K. and Shyam, K.R. 1987. Effect of nutrients and leguminous plant extracts on spore germination of Colletotrichum lindemuthianum, the agent of bean anthracnose. Indian J. Mycol. Plant Pathol. 17: 345-347.
  • Chakrabarty, P.K. and Shyam, K.R. 1988. Evaluation of systemic fungitoxicants against Colletotrichum lindemuthianum, the incitant of bean anthracnose. Indian J. Plant Pathol. 6: 67-70.
  • Chakrabarty, P.K. and Shyam, K.R. 1989. Host range studies with three isolates of Colletotrichum lindemuthianum, causing anthracnose of bean (Phaseolus vulgaris L.). Indian J. Mycol. Pl. Pathol. 19: 14-17.
  • Chakrabarty, P.K. and Shyam, K.R. 1989. Meteorological factors affecting Curd Rot of Cauliflower. Indian J. Mycol. Pl. Pathol. 19: 55-59.
  • Chakrabarty, P.K. and Shyam, K.R. 1989. Some new records of microorganisms associated with Curd rot of Cauliflower. Indian J. Mycol. Pl. Pathol. 19: 118-120.
  • Chakrabarty, P.K. and Shyam, K.R. 1990. Reactions of some promising lines of french bean to different isolates of Colletotrichum lindemuthianum in Himachal Pradesh. Indian J. Plant Pathol. 8: 86-88.
  • Chakrabarty, P.K. and Shyam, K.R. 1992. Effect of frost, boron and different levels of nitrogen on Curd Rot of cauliflower. Indian Phytopath. 45: 237-239.
  • Chakrabarty, P.K. and Taneja, N.K. 1990. Effect of nutrients on Curd Rot of Cauliflower (Brassica oleracea convar botrytis var. botrytis). Indian J. Agric. Sci. 60: 74-76.
  • Chakrabarty, P.K and D.W. Gabriel. 1995. Plasmid-borne pathogenicity genes of Xanthomonas campestris pv. malvacearum. Phytopathology 85: 1156.
  • Chakrabarty, P.K., Duan, Y.P. and Gabriel, D.W. 1997. Cloning and characterization of a member of the Xanthomonas avr/pth gene family member that defeats all commercially available R genes in cotton in USA . Phytopathology 87: 1160-1167.
  • Chakrabarty, P.K., Mahadevan, A., Raj, S., Meshram, M.K. and Gabriel, D.W. 1995. Plasmid borne determinants of pigmentation, exopolysaccharide production, and virulence in Xanthomonas campestris pv. malvacearum . Can. J. Microbiol. 41: 740-745.
  • Chakrabarty, P.K., Meshram, M.K. and Raj, S. 1992. Outbreak of Die-back and Canker in Silk Cotton (Ceiba pentandra (L.) Gaertn.). Indian J. Agric. Sci. 63: 599- 601.
  • Chakrabarty, P.K., Mukewar, P.M., Raj, S. and Sravan Kumar, 2002. Biochemical factors governing resistance in diploid cotton against grey-mildew disease. Indian Phytopath. 55(2): 69-75.
  • Chakrabarty, P.K., Raj, S. and Taneja, N.K. 1991. A new record of late Phoma blight of cotton (Gossypium species). Indian J. Agric. Sci. 61: 223-224.
  • Chakrabarty, P.K., Singh, B.M. and Shyam, K.R. 1989. Physiologic Specialization in Colletotrichum lindemuthianum causing anthracnose of french bean in higher hills of Himachal Pradesh , India . Indian J. Plant Pathol. 7: 55-60.
  • Mukewar, P.M., Raj, S. and Chakrabarty, P.K. 1994. Epidemic occurrence of grey mildew disease of cotton in Central India . J. Indian Soc. Cot. Improv. 19: 170-173.
  • Mukewar, P.M., Raj, S. and Chakrabarty, P.K. 1994. Variability of symptoms in cotton hybrids by Ramularia areola. J. Cot. Res. and Dev. 8: 200-201.
  • Gabriel D.W., Yuan, Q., Yang, Y. and Chakrabarty, P.K. 1997. Role of nuclear localizing signal sequences in three disease phenotypes determined by the Xanthomonas avr/pth gene family. Mol Plant-Microbe Interact.
  • Nodzon, L., Xu, W.H., Wang, Y., Pi, L., Chakrabarty, P.K., Song, W.Y. 2004. The ubiquitin ligase Xbat-32 regulates lateral root development in Arabidopsis. Plant Journal 40: 996-1006.
  • Raj, S., Chakrabarty, P.K. and Meshram, M.K. 1990. Epidemiology of and loss caused due to New Wilt of Cotton. Indian J. Plant Pathol. 8: 23-27.
  • Raj, S., Chakrabarty, P.K. and Meshram, M.K. 1990. New Wilt and Drying of Cotton in India- comparative observations. J. Cotton. Res. and Dev. 4: 65-69.
  • Raj, S., Chakrabarty, P.K., Meshram, M.K. and Bambawale, O.M. 1991. Investigations on New Wilt of Cotton. Cot. et Fib. Trop. 46: 207-215.
  • Shyam K.R. and Chakrabarty, P.K. 1985. Seed transmission of Colletotrichum lindemuthianum in different cultivars of bean (Phaseolus vulgaris L.). Seed Research 13: 211-212.
  • Shyam K.R., Chakrabarty, P.K., Bhardwaj, S.S. and Dohroo, N. 1987. Pythium Curd Rot of Cauliflower. Indian J. Plant Pathol. 5: 118.
  • Chakrabarty, P. K., Sable, S., Monga. D. and Mayee, C.D. 2005. Polymerase chain reaction based detection of Xanthomonas axonopodis pv malvacearum and cotton leaf curl virus. Indian J. Agric. Sci. 75 (8):524-27.
  • Chakrabarty, P.K. and Mayee, C.D. 2004. Recent Approaches to Manage Diseases of Cotton. J. Indian Soc. Cotton Improv. 29:65-79.
  • Monga, D., Rathore S. S., Sethi, R., Saini, M. and Chakrabarty P. K. 2005. Rapid preparation of DNA from Rhizoctonia solani and R. bataticola fungi causing root rot in cotton. J. Cot. Res & Dev. 19: 84-87 .
  • Wang Young-Sheng, Pi, Li., Chen, X., Chakrabarty, P. K., Jiang, J., Leon, A., Lui, G. Z., Li, L., Benny, U., Oard, J., Ronald, P. C. and Song, W. Y. 2006. Rice XA21binding protein 3 is a Ubiquitin Ligase required for full Xa21- mediated disease resistance. The Plant Cell 18: 3635-46.
  • Chavhan, R.L., Chakrabarty, P.K., Patil, F. S., Patil, H. B., Singh, S.K. and Khadi, B. M. 2008. Association of new fungal species with leaf spot and blight of Sunflower and cloning of their ribosomal RNA genes. Indian Phytopathology 61:70-74.
  • Monga, D., Kumar, M., and Chakrabarty, P.K. 2008. Use of an rRNA Internal Transcribed Spacer Region to Distinguish Backly Related Isolates of the Genera Rhizoctonia . Indian J. Genet., 68 (3): 307-312.
  • Kadam, B.P., Chavhan, R.L., Chakrabarty, P.K. and Patil, F.S. 2009. Characterization of variability in some economically important species of Alternaria based on the nucleotide sequences of nuclear ribosomal DNA. J. Plant Biochemistry & Biotechnology 18:59-64.
  • Nandeshwar, S.B., Moghe, S, Chakrabarty, P.K., Deshattiwar, M.K., Kranthi, K., Anandkumar, P., Mayee, C.D. and Khadi, B.M. 2009. Agrobacterium-mediated transformation of cry 1Ac Gene into Shoot Tip Meristem of Diploid Cotton Gossypium arboreum cv. RG 8. Plant Mol Biol Reptr 27 (4): 549-557.
  • P K Chakrabarty, R L Chavhan, A Ghosh and D W Gabriel. 2009. Rapid and Efficient Protocols For Throughput Extraction of High Quality Plasmid DNA from Strains of Xanthomonas axonopodis pv. malvacearum and Escherichia coli . J. Plant Biochemistry & Biotechnology 19 : ( published on line Nov 10, 2009 ).

B. Book Chapters / Reviews

  • Chakrabarty, P.K. and Bambawale, O.M. 2004. Current trends in use of Molecular and Novel Approaches in Plant Disease Management. In Advances in Plant Sciences (Eds. D. Prasad and Amerika Singh), Akansha Publishing House, New Delhi . pp. 45-61.
  • Chakrabarty, P.K. and Mayee, C.D. 2004. Recent Approaches to Manage Diseases of Cotton. J. Indian Soc. Cotton Improv. 29:65-79.
  • Mayee, C.D . and Chakrabarty, P.K. 2004. Biotechnological Approaches for Integrated Management of Plant Diseases. In: Biotechnological Approaches for Integrated Management of Crop Diseases (Eds. C.D. Mayee, C. Manoharachary, K.V.R.R. Tilak, D.S. Mukadam, J. Deshpande). Daya Publishing House, New Delhi , pp. 45-73.
  • Chakrabarty, P.K., and Raj, S. 2002. Molecular analysis of avirulence gene with special reference to Plant Pathogenic Xanthomonads. In Molecular Approaches in Plant Disease Management (Eds. G.S. Shekhawat, B.P. Singh and R.A. Singh). Malhotra Publishing House, New Delhi , pp 63-87.
  • Chakrabarty, P.K., Gabriel, D.W. and Raj, S. 1996. Cloning and characterization of a pathogenicity gene from a highly virulent strain of Xanthomonas campestris pv. malvacearum . In : Plant pathogenic bacteria, (ed., A. Mahadevan). IX International Conference on Plant Pathogenic Bacteria, Aug. 26-29, 1996, University of Madras , Madras , India , Udayan offset, Chennai, pp. 236-246.
  • Chakrabarty, P.K., Gabriel, D.W., Sravan Kumar, V. and Raj, S. 2001. Molecular basis of pathogenicity of Xanthomonas axonopodis pv . malvacearum and use of cloned pathogenicity genes as diagnostic tools. In: Role of Resistance in Intensive Agriculture (eds: S. Nagarajan and D.P. Singh), Kalyani Publishers, New Delhi , India . pp.115-135.
  • Gabriel D.W., Q. Yuan, Y. Yang, and P.K. Chakrabarty . 1997. Role of nuclear localizing signal sequences in three disease phenotypes determined by the Xanthomonas avr/pth gene family: Bacterial Determinants for Pathogenicity and Avirulence. In: Biology of Plant-Microbe Interactions, Volume 1 (Eds: Gary Stacey, Beth Mullin, and Peter M. Gresshoff. International Society for Molecular Plant-Microbe Interactions.
  • Raj, S., Chakrabarty, P.K. and Monga, D. 2001. Cotton leaf curl virus: present status and future strategies for its management. In: IPM System in Agriculture (eds: R.K. Upadhyay, K.G. Mukerji and O.P. Dubey), Vol. 8, Key Pathogens and Diseases, Aditya Books Pvt. Ltd., New Delhi, India, pp. 471-496.
  • Raj, S., Meshram, M.K. and Chakrabarty, P.K. 1999. Major diseases of cotton, their management options and strategies. In: IPM System in Agriculture (eds: R.K. Upadhyay, K.G. Mukerji and O.P. Dubey), Vol. 6, Cash crops, Aditya Books Pvt. Ltd., New Delhi, India, pp.123-164.
  • Chakrabarty, P.K., Chatterjee, A, Gassmann, W. and Chatterjee, A.K. 2004. Bacterial products important for plant disease- Cell surface components. In Encyclopedia of Plant and Crop Science, R.M. Goodman (Ed.). Marcel Dekker Inc., New York , pp. 92-94.
  • Chakrabarty, P.K., Chatterjee, A, Gassmann, W. and Chatterjee, A.K. 2004. Bacterial products important for plant disease- Extracellular enzymes and secretory system. In Encyclopedia of Plant and Crop Science, R.M. Goodman (Ed.). Marcel Dekker Inc., New York , pp. 98-100.
  • Chakrabarty, P.K., Chatterjee, A, Gassmann, W. and Chatterjee, A.K. 2004. Bacterial products important for plant disease- Toxins and Growth factors. In Encyclopedia of Plant and Crop Science, R.M. Goodman (Ed.). Marcel Dekker Inc., New York , pp. 101-104.
  • Chakrabarty, P.K., Chatterjee, A, Gassmann, W. and Chatterjee, A.K. 2004. Bacterial products important for plant disease- Diffusible metabolites. In Encyclopedia of Plant and Crop Science, R.M. Goodman (Ed.). Marcel Dekker Inc., New York , pp. 95-97.
  • Chakrabarty, P.K. 2003. Molecular diagnostics and development of transgenics in relation to plant diseases. Proccedings “International Seminar on Sugarcane Genomics and Genetic Transformation”, Vasantdada Sugar Institute, Pune, August 28-29, 2003. pp. 39-47.
  • Chakrabarty, P.K., Chavhan, R. L. and Dhanole, Rita. 2008. Bacterial blight/Angular leaf spot of Cotton. In : Handbook of Crop Diseases- Identification and Management (Eds. L.V. Gangawane and V.C. Khilare), Daya Publishing House, New Delhi, p. 75-76.
  • Chakrabarty, P.K., Chavhan, R. L. and Dhanole, Rita. 2008. Grey mildew of Cotton. In : Handbook of Crop Diseases- Identification and Management (Eds. L.V. Gangawane and V.C. Khilare), Daya Publishing House, New Delhi, p. 77-78.
  • Chakrabarty, P.K., Chavhan, R. L. and Dhanole, Rita. 2008. Root rot of Cotton. In : Handbook of Crop Diseases- Identification and Management (Eds. L.V. Gangawane and V.C. Khilare), Daya Publishing House, New Delhi, p. 79-80.
  • Chakrabarty, P.K., Chavhan, R. L. and 2008. Leaf Curl Virus of Cotton. In : Handbook of Crop Diseases- Identification and Management (Eds. L.V. Gangawane and V.C. Khilare), Daya Publishing House, New Delhi, p. 81-82.

C. Conference papers/ Abstracts

  • Chakrabarty, P.K. 2003. Molecular diagnostics and development of transgenics in relation to plant diseases. In: Proccedings “International Seminar on Sugarcane Genomics and Genetic Transformation”, Vasantdada Sugar Institute, Pune, August 28-29, 2003. pp. 39-47.
  • Chakrabarty, P.K. 2004. Cloning Genes for Pest Resistance in Plants. In: Winter School on Application of Molecular Biology in Pest management, sponsored by ICAR, PAU Ludhiana, Dec 1-11, 2004. pp. 1-16.
  • Chakrabarty, P.K. 2004. Plant disease diagnostics and cloning of resistance gene analogs with special reference to cotton and its pathogens. In: International Symposium on “strategies for sustainable cotton production- A global Vision. UAS Dharwad , India , Nov. 23-25, 2004. 1. Crop Improvement. Pp. 373-383.
  • Chakrabarty, P.K. 2004. Serological and molecular detection of cotton leaf curl virus. In: Proceedings of National Seminar on cotton leaf curl virus disease, CICR, Regional Station, Sirsa, December, 15, 2004. pp 3-8.
  • Chakrabarty, P.K., Gabriel, D.W. and Raj, S. 1996. Cloning and characterization of a pathogenicity gene from a highly virulent strain of Xanthomonas campestris pv. malvacearum. In: Plant pathogenic bacteria, (ed., A. Mahadevan). IX International Conference on Plant Pathogenic Bacteria, Aug. 26-29, 1996, University of Madras , Madras , India , Udayan offset, Chennai, pp. 236-246.
  • Monga, D., Jeya Kumar, Chakrabarty, P.K. 2004. Epidemiology of cotton leaf curl virus disease. In: Proceedings of National Seminar on cotton leaf curl virus disease, CICR, Regional Station, Sirsa, December, 15, 2004. pp. 43-50.
  • Raj, S., Chakrabarty, P.K. and Monga, D. 1996. Status of cotton diseases and advancement in management research. Century of Cotton in India , Main Cotton Research Station, Gujarat Agricultural University, Surat , Dec. 21, 1996, 60-65pp.
  • Chakrabarty, P.K and D.W. Gabriel. 1995. Plasmid-borne pathogenicity genes of Xanthomonas campestris pv. malvacearum. Phytopathology 85: 1156.
  • Chakrabarty, P.K. 1998. Plasmid-borne pathogenicity attributes of Xanthomonas axonopodis pv. malvacearum and cloning and characterization of pathogenicity genes from its highly virulent strain. Fiftieth Annual Meeting of the Indian Phytopathological Society and the National Symposium on present scenario in diseases of oilseeds and pulses, Feb. 17-19, 1998, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India, pp. 67-69.
  • Chakrabarty, P.K. and Gabriel D.W. 2004. Initiatives in cloning bacterial blight resistance genes from cotton. International Cotton Genome Initiative ICGI-2004 workshop, Taj Krishna, Hyderabad , Oct 10-13, 2004, p. 52. (Abstract).
  • Chakrabarty, P.K. and Gabriel, D.W. 1996. Sequence analysis of Xanthomonas avr/pth gene family member that defeats all known cotton R genes. Plant Molecular and Cellular Biology Workshop, March 30-31, 1996, Whitney Lab., Univ. of Florida , Marineland , USA .
  • Chakrabarty, P.K. and Gabriel, D.W. 2004. Molecular approaches for detection and differentiation of strains of Xanthomonas axonopodis pv. malvacearum and initiatives in cloning bacterial blight resistance genes from cotton. National symposium on Crop Surveillance: Disease forecasting and Management, IARI, New Delhi , Feb 19-21, 2004, pp.2-3 (Abstract).
  • Chakrabarty, P.K. and Raj, S. 1994. Plasmids of Xanthomonas campestris pv. malvacearum and their role in the most virulent race 18. World Cotton Conference-1. Challenging the future. Brisbane , Australia , February 14-17, 1994, p. 96 (Abstr.).
  • Chakrabarty, P.K. and Raj, S. 2000. Cloning and characterization of the plasmid borne pathogenicity genes of Xanthomonas axonopodis pv. malvacearum and their use as diagnostic tools. Fifty second Annual Meeting of the Indian Phytopathological Society and the role of resistance in intensive agriculture, Feb. 17-19, 2000, Directorate of Wheat Research, Karnal , India , pp. 7-8.
  • Chakrabarty, P.K., Gabriel, D.W. and Raj, S. 1997. Pathogenicity gene pthN in Xanthomonas axonopodis pv. malvacearum pleiotropically encodes hyper-sensitive response in bacterial blight resistant cotton (G.hirsutum). Golden Jubilee International Conference on Integrated Plant Disease Management for Sustainable Agriculture, Nov. 10-15, 1997, IARI, New Delhi , p. 135.
  • Chakrabarty, P.K., Ghosh, A., Meshram, M.K., Wasule, D.L., Raj, S. and Mayee, C.D. 2002. Molecular diagnostic tools for detection and race-differentiation of Xanthomonas axonopodis pv. malvacearum strains. Annual Meeting of Indian Society of Plant Pathologists and Conference on perspectives in Integrated Plant Disease Management, NRCC, Nagpur, February, 13-14, 2002.
  • Chakrabarty, P.K., Mahadevan, A., Raj, S. and Meshram, M.K. 1992. Plasmid profiles and curing of plasmids in Xanthomonas campestris pv. malvacearum strains isolated from cotton. Symposium on Frontiers in Plant Biotechnology, 25-27 Nov. 1992, IARI, New Delhi , India .
  • Chakrabarty, P.K., Mayee, C.D., Sable, S.V., Banerjee, B. and Raj, S. 2004. DNA based diagnostic tools for precise detection and differentiation of strains of Xanthomonas axonopodis pv. malvacearum and cotton leaf curl Gemini virus, two economically important pathogens of cotton. National symposium on Crop Surveillance: Disease forecasting and Management, IARI, New Delhi , Feb. 19-21, 2004, p. 35 (Abstract).
  • Chakrabarty, P.K., Raj, S. and Kairon, M.S. 1997. Cultivar-specific pathogenicity symptoms of Xanthomonas campestris pv. malvacearum is governed by the 102-bp tandem repeats of pthN. Symposium on the role of Plant Biochemistry and Biotechnology in Improving Crop Productivity, March 18-20, 1997, ICAR Research Complex for NEH region, Barapani, India, p. 38 (Abstr.).
  • Chakrabarty, P.K., Raj, S. and Meshram, M.K. 1992. Biochemical basis of bacterial blight resistance in cotton (Gossypium hirsutum). Silver Jubilee Conference of AICCIP, 17-19 Sept. 1992, Coimbatore , India .
  • Chakrabarty, P.K., Raj, S. and Mukewar, P.M. 1993. Phenol, carbohydrate and amino acid metabolism in grey mildew (Ramularia areola) susceptible and resistant diploid cotton. National Symposium on Innovative Approaches in Plant Disease Management, 25-27 Nov. 1993, P.A.U., Ludhiana , India .
  • Chakrabarty, P.K., Sable, S.V, Monga, D., and Mayee, C.D. 2005. Cloning and expression of coat protein gene of cotton leaf curl virus for use as highly specific immunogen and development of a twig imprint blot method for detection of the virus. National Symposium on Crop Disease Management in Dryland Agriculture, MAU, Parbhani, Feb 12-14, 2005.
  • Chavhan, R.L., Chakrabarty, P.K., Patil, F.S., Mayee, C.D. 2005. Molecular characterization of Alternaria complex causing leaf spot and blight of Sunflower. National Symposium on Crop Disease Management in Dryland Agriculture, MAU, Parbhani, Feb 12-14, 2005.
  • Gabriel, D.W., Yuan, Q., Yang, Y. and Chakrabarty, P.K. 1996. Role of nuclear localizing signal sequences in three disease phenotypes determined by the Xanthomonas avr/pth gene family. Eighth International Congress on Molecular Plant -Microbe Interactions, July 14-19, 1996, Knoxville , Tennessee , USA.
  • M. K. Meshram, P.K. Chakrabarty, C. D. Mayee, Sheo Raj, A.S. Ghosh & K. N. Gupta. 2001. Development of sensitive molecular diagnostic tools for detection of Xanthomonas axonopodis pv. malvacearum races. National Seminar in Hindi on Sustainable Cotton Production Technologies and Future Strategies. CICR, Nagpur , Sept. 10-11, 2001, Souvenir, p. 114 (Abstracts).
  • Raj, S. and Chakrabarty, P.K. 1994. Disease management technology in cotton. Seminar on Present Status and Future Thrusts in Cotton Production in India . Bombay , May 7-8, 1994, 30 pp.
  • Raj, S., Chakrabarty, P.K. and Monga, D. 1994. Future options for the management of cotton diseases. World Cotton Conference-1, challenging the future, Feb. 14-17, Brisbane , Australia , 1994, p.100 (Abstract).
  • Raj, S., Chakrabarty, P.K. and Monga, D. 1997. Technological innovations in disease management for eco-friendly and sustainable cotton production. Golden Jubilee International Conference of the Indian Phytopathological Society on Integrated Plant Disease Management for Sustainable Agriculture, Nov. 10-15, 1997, IARI, New Delhi , p. 106.
  • Sheo Raj, K.N. Gupta, P.K. Chakrabarty, A.S. Ghosh and D. Monga. 2001. Standardisation of protocol for isolation of high molecular weight genomic DNA from cotton plant. National Seminar in Hindi on Sustainable Cotton Production Technologies and Future Strategies. CICR, Nagpur , Sept. 10-11, 2001, Souvenir, p. 118 (Abstracts).
  • Sheo Raj, K.N. Gupta, P.K. Chakrabarty, S.K. Banerjee, P. Jeyakumar and D. Monga. 2001. A new protocol developed for isolation of genomic DNA from Whitefly (B. tabaci Genn). National Seminar in Hindi on Sustainable Cotton Production Technologies and Future Strategies. CICR, Nagpur , Sept. 10-11, 2001, Souvenir, p. 116 (Abstracts).
  • Chakrabarty, P.K., Sable, S.V, Monga, D., and Mayee, C.D. 2005. Cloning and expression of coat protein gene of cotton leaf curl virus for use as highly specific immunogen and development of a twig imprint blot method for detection of the virus. National Symposium on Crop Disease Management in Dryland Agriculture, MAU, Parbhani, Feb 12-14, 2005. pp. 11-12.
  • Chavhan, R.L., Chakrabarty, P.K., Patil, F.S., Mayee, C.D. 2005. Molecular characterization of Alternaria complex causing leaf spot and blight of Sunflower. National Symposium on Crop Disease Management in Dryland Agriculture, MAU, Parbhani, Feb 12-14, 2005.
  • Chakrabarty, P.K. 2005. Molecular approaches for pathogen diagnosis and cloning genes involved in host resistance. Global conference-II, RCA, MPUAT, Udaipur , Nov. 25-29, 2005. pp. 280-281.
  • Chakrabarty, P. K. 2006. Recent advances in diagnosis and management of plant diseases in the era of molecular Plant Pathology. Presidential Lecture (West Zone). National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp.25-26.
  • Chakrabarty, P.K. Chavhan, R.L., Suchitra Sable, Monga, D. and Khadi. B.M. 2006. Molecular detection and diagnosis of economically important fungal pathogens of cotton. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp. 41-42.
  • Chakrabarty, P.K. Narwade, A.V. Chavhan, R.L., Suchitra Sable and Khadi, B.M. 2006. Development of molecular diagnostic methods for identification of Ramularia areola strains and differentiation of isolates infecting four cultivated Gossypium species. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp.39.
  • Chakrabarty, P.K., Chavhan, R.L., Monga D. and Sinha. D.P. 2006. PCR based detection of Xanthomonas cymopsidis the Bacterial Blight pathogen of Guar ( Cymopsidis tetragonaloba L.). National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp.41.
  • P.K. Chakrabarty, R.L. Chavhan, Suchitra Sable and L.A. Deshpande 2006. RFLP marker for diagnosis of race 18 strains of Xanthomonas axonopodis pv. malvacearum. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, 42-43.
  • Chavhan, R.L., Chakrabarty, P.K., Patil, F.S. and Khadi, B.M. 2006. Molecular diagnosis of economically important fungal pathogens of crop plants. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp. 30
  • Singh, Pawan Kumar, Chakrabarty, P. K. and Pande. S. S. 2006. Morphological and Molecular Characterization of Rhizoctonia species causing root rot of commercially important crops. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp.39-40.
  • Chavhan, R.L., Chakrabarty, P.K., Patil, F. S., Patil, H. B. and Singh, S.K. 2007. Association of new fungal species with leaf spot and blight of Sunflower and cloning of their ribosomal RNA genes. National symposium on recent trends in diagnosis and management of chronic and emerging plant diseases, NBSS&LuP, Nagpur . Nov 23 -24, 2006, pp.40.
  • Chakrabarty, P.K., Chavhan, R. L. Sable, Suchitra, Narwade, A. V., Monga, D. and Khadi B.M. 2007. Detection of four economically important fungal pathogens of cotton using PCR and restriction enzyme analysis. World Coton Research Conference-4, Lubbock , Texas , Sept 10-14, 2007.
  • Chakrabarty, P.K. 2007. Molecular approaches in diagnosis and management of plant diseases. In: National Symposium on Potentials of Bio-Control agents in Agriculture Prospects and Perspectives, Society of Mycology and Plant Pathology, College of Agriculture, Nagpur, Oct 27 -28, 2007.
  • Chavhan, R.L. Chakrabarty P.K. and Mohan K.S. 2007. Molecular characterization of diversity among race 18 strains of Xanthomonas axonopodis pv. malvacearum . In: National Symposium on Potentials of Bio-Control agents in Agriculture Prospects and Perspectives, Society of Mycology and Plant Pathology, College of Agriculture, Nagpur, Oct 27 -28, 2007.
  • Chakrabarty, P.K. 2007. Diagnosis of Plant Pathogens- Novel Approaches. In: National Symposium on Plant Protection-Technology Interface, Association for Advancement in Plant Protection, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, W. Bengal, 28-29th Dec, 2007.
  • Narwade, A.V., Chakrabarty, P.K. and Mohan, K.S. 2008. Molecular diagnosis of Ramularia areola and characterization of variability among trains infecting four cultivated species of cotton using restriction fragmentation and RAPD markers. International Conference on Toxic Exposure related Biomarker: Genomes & Health Effects, National Environmental Engineering Research Institute, Nagpur , Jan 10-11, 2008.
  • Chakrabarty, P.K. 2009. Molecular diagnosis of plant diseases. Fifth international conference on Plant Pathology in the Global era, organised by Indian Phytopathological Society, at IARI, Nov 10-13, 2009.
  • Chakrabarty, P.K. 2009. Molecular approaches in diagnosis of cotton diseases. National Seminar of Bt-Cotton- Opportunities and Prospects, organised by Cotton Research and Development Association, Hisar, at Central Institiute for Cotton, Nov 17-19, Nagpur. 2009.

Best Paper Award

  • Chakrabarty, P.K., Mayee, C.D., Sable, S.V., Banerjee, B. and Raj, S. 2004. DNA based diagnostic tools for precise detection and differentiation of strains of Xanthomonas axonopodis pv. malvacearum and cotton leaf curl Gemini virus, two economically important pathogens of cotton. National symposium on Crop Surveillance: Disease forecasting and Management, IARI, New Delhi , Feb. 19-21, 2004, p. 35
  • Chakrabarty, P.K., Sable, S.V, Monga, D., and Mayee, C.D. 2005. Cloning and expression of coat protein gene of cotton leaf curl virus for use as highly specific immunogen and development of a twig imprint blot method for detection of the virus. National Symposium on Crop Disease Management in Dryland Agriculture, MAU, Parbhani, Feb 12-14, 2005. pp. 11-12.
  • Chavhan, R.L. Chakrabarty, P.K. and Mohan K.S. 2007. Molecular characterization of diversity among race 18 strains of Xanthomonas axonopodis pv. malvacearum . In: National Symposium on Potentials of Bio-Control agents in Agriculture Prospects and Perspectives, Society of Mycology and Plant Pathology, College of Agriculture, Nagpur, Oct 27 -28, 2007.
  • Narwade, A.V., Chakrabarty, P.K. and Mohan, K.S. 2008. Molecular diagnosis of Ramularia areola and characterization of variability among trains infecting four cultivated species of cotton using restriction fragmentation and RAPD markers. International Conference on Toxic Exposure related Biomarker: Genomes & Health Effects, National Environmental Engineering Research Institute, Nagpur , Jan. 10-11, 2008.

E. ICAR / CICR News letter

  • Chakrabarty, P.K. 1999. Pathogenicity genes cloned from Xanthomonas campesrtis pv. malvacearum as diagnostic tools. ICAR News 5: 1-3.
  • Chakrabarty, P.K. 2003. Development of DNA based diagnostic tools for detection of strains of Xanthomonas axonopodis pv. malvacearum . CICR Newsletter XIX (1): 8-9.
  • Chakrabarty, P.K. 2003. PCR based detection of Xanthomonas axonopodis pv. malvacearum and cotton leaf curl virus, two major pathogens of cotton. CICR Newsletter XIX (2): 4-5.
  • Chakrabarty, P.K. and Monga Dilip. 2004. Diagnostic tools for detection of Xanthomonas axonopodis and leaf curl virus on cotton. ICAR News 10: 1-4.
  • Chakrabarty, P . K. 2006. Diagnosis of Plant Pathogens- Novel Approaches. Science India 9(12) 7-10.

F.Training Manual

  • Chakrabarty, P.K. 2004. Techniques in Molecular Plant Pathology- Training Manual, Crop Protection Division, Central Institute for Cotton Research, Nagpur , 34 pp.

G.Research reports

  • Chakrabarty, P.K. 1992. Molecular basis of Pathogenicity and race-specificity in Xanthomonas campestris pv. malvacearum with reference to extra-chromosomal genetic elements. Center of Advanced Study in Botany, University of Madras , under the SERC Visiting Scientist scheme of DST, submitted to the DST, New Delhi , 21 pp.
  • Chakrabarty, P.K. 1996. Molecular basis of pathogenicity and host-specificity of Xanthomonas campestris pv. malvacearum , the causal agent of bacterial blight of cotton. Report of the work done at the University of Florida , Gainesville , USA , under Overseas Associateship Program of DBT, submitted to the DBT, New Delhi , 59 pp.
  • Chakrabarty, P.K. 2003. Development of sensitive Molecular diagnostic tools for rapid detection and differentiation of races of Xanthomonas axonopodis pv. malvacearum . Final Report, submitted to DBT, New Delhi , 45 pp.
  • Chakrabarty, P.K. 2004. Development of Diagnostic tools for differentiation of biotypes/ races of pathogens and insect pests of cotton. Annual Report of TMC MMI (MM3.2), 2003-04, ICAR New Delhi , 70 pp.
  • Chakrabarty, P.K. 2007. Development of Diagnostic tools for differentiation of biotypes/ races of pathogens and insect pests of cotton. Annual Report of TMC MMI (MM3.2), 2003-04, ICAR New Delhi , 70 pp.
  • Chakrabarty, P.K. 2008. DNA based diagnostic methods to identify biotype diversity in race 18 of Xam and commercialization of a PCR detection kit. Report submitted to DBT, New Delhi , 25 pp.
  • Chakrabarty, P.K. 2008. Engineering virus resistant cotton through dsRNAi-mediated targeting of cotton leaf curl virus. Report submitted to DBT, New Delhi , 17 pp.